Figure 5.

 Sequences of the protein subunits have little similarity to bacterial or mitochondrial RNase P proteins. The amino acid sequences of the five newly identified subunits are shown, as derived from the SGD (http://genome-www.stanford.edu/Saccharomyces/). Of the five ORFs, only POP8 contains a predicted intron, composed of 75 bp between the first and second base pair of the sixteenth codon of the ORF. PCR analyses using primers flanking the intron with genomic DNA and cDNA library confirm that the POP8 cDNA gives a smaller PCR product by the expected amount. As discussed in the text, these sequences do not display strong similarities to other gene sequences in current databases and do not display notable similarities when aligned pairwise with each other or with other identified RNase P or RNase MRP proteins. Sequence features that are discussed in the text are indicated by underlining (lysines and arginines) or boldface type (lysine residues that form a short pattern in all RNase P subunits except Rpp1p).