Figure 5.

 Chromatin structure of the S. cerevisiae 753-bp RE fragment in the mcm1-5 mutant strain grown at 37°C mapped by primer extension analysis of micrococcal nuclease cleavage sites using primer a290. MATa and MATα cells (CWU116 and CWU117) are as indicated. Extensions of undigested chromatin (0) and chromatin digested using two or three levels of micrococcal nuclease are presented. (D) Protein-free DNA digests. Coordinates refer to the published sequence of chromosome III. The Mcm1–Matα2 binding site (α2) is represented by a shaded box. DNA regions containing TTT(A/G) repeats are depicted in open boxes.