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. Author manuscript; available in PMC: 2012 Sep 13.
Published in final edited form as: Biochemistry. 2011 Aug 16;50(36):7787–7799. doi: 10.1021/bi201060c

Figure 5.

Figure 5

Stimulation of Ca2+ release by Ang II and PHA results in sGC inhibition. Jurkat T cells (0.5 × 106) were incubated with 5 µM BAPTA-AM or vehicle control (DMSO) 15 min prior to the addition of indicated concentrations of PHA, Ang II (1 µM) or buffer and an additional 2 min incubation, followed by addition of DEA/NO (10 µM). The reaction was stopped after 2 min. Both PHA and Ang II inhibited sGC in the absence of BAPTA, but not in its presence. A. PHA. B. Ang II. Error bars represent the standard deviation from the mean of independent experiments (n = 5), and * denotes p < 0.01.