Figure 2.

3D Polystyrene scaffold for 3D coculture model. Polystyrene fibers are laid down at 90° angles with respect and offset from each other, creating its characteristic 3D structure. There is spacing between each fiber (white arrow) to create the interconnected porous structure of the scaffold. The scaffold is made with fibers 300-um in diameter and 400-um spacing between the fibers (a, b). The scaffold also has a loop around the rim of the scaffold. The loop is about 0.5-mm height (black arrows) allowing the scaffold to have a raised conformation with reference to the bottom of the well (c, d).