Table 2.
RuvAB directs the orientation of RuvC-mediated Holliday junction resolution
|
A X1 orientation
|
Resolution (%)
|
Fold stimulation
|
||||
|---|---|---|---|---|---|---|
|
C
|
BC
|
ABC
|
C
|
BC
|
ABC
|
|
| 2/4 | 2.6 | 10.1 | 12.8 | 1 | 3.9 | 4.9 |
| 1/3
|
1.1
|
1.2
|
0.3
|
1
|
1.1
|
0.3
|
| B X2 orientation | ||||||
| 2/4 | 1.9 | 2.3 | 0.4 | 1 | 1.2 | 0.2 |
| 1/3 | 1.3 | 6.9 | 12.9 | 1 | 5.3 | 9.9 |
The data shown in Fig. 3, produced by the cleavage of junctions X1 or X2 with RuvC, RuvBC, or RuvABC, was quantified by PhosphorImaging. Resolution in orientations 2/4 or 1/3 is expressed as a percentage of total resolution and relative to cleavage by RuvC alone.