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. 1999 Jul 15;13(14):1794–1806. doi: 10.1101/gad.13.14.1794

Figure 6.

Figure 6

AWB mediates attractive chemosensory behaviors in lim-4. Graphic representation of single worm chemotaxis assays: Strains and neurons in those strains that express ODR-10 are at left, and assay results are at right. −6 indicates complete repulsion from diacetyl, 0 indicates indifference, and +6 indicates attraction. Each dot represents the chemotaxis score for a single worm, and vertical lines indicate the median score. All strains tested also carried a mutation in the lin-15 marker and were rescued with an extrachromosomal lin-15 gene. Assays were performed with (A) lim-4(ky403) mutants, (B) lim-4 odr-10(ky225) double mutants, (C) lim-4 odr-10 animals with extrachromosomal str-2::ODR-10 and str-2::GFP (only animals expressing GFP in AWC and AWB were assayed), (D) the same strain as in C following laser ablation of the GFP-expressing AWC neuron, (E) an odr-10 strain wild-type for LIM-4, expressing the str-2::ODR-10 and str-2::GFP transgenes, and F the same strain as in E following laser ablation of the GFP-expressing AWC neuron. (G) Data from a str-1::ODR-10 strain in a wild-type background, reproduced from Troemel et al. (1997) for comparison. Data were analyzed with a Mann-Whitney rank sum test, and significant differences (P ≤ 0.002) were observed between D and G, B and C, B and D, D and F, and C and E. D and E were not significantly different (P = 0.499).