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. 1999 Jul 15;13(14):1884–1897. doi: 10.1101/gad.13.14.1884

Figure 7.

Figure 7

Figure 7

Ectopic expression of hnRNP D in proliferating K562 cells has little effect on decay of the ARE-containing mRNA. Transfection of K562 III-2 cells, RNA extraction, and RNA blot analysis were carried out as described in the legend to Fig. 1. (A) RNA blots showing decay of the β-globin (BBB) mRNA bearing the fos ARE in the presence (left) or absence (right) of the p42 isoforms in proliferating cells. (B) (Left) Graph showing kinetics of decay of the BBB+AREfos mRNA in proliferating cells overexpressing individual isoforms of hnRNP D. Data collection and analysis are as described in Fig. 2. Symbols are depicted as follows: (Solid oval) Vector alone or control; (solid square) p37; (solid diamond) p40; (x) p42; (+) p45. (Right) Western blot analysis for exogenously expressed myc-tagged hnRNP D was performed as described in the legend to Fig. 6. Cytoplasmic lysates were prepared from proliferating cells transfected with cDNAs for individual isoforms of hnRNP D.