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. 2011 Aug 30;6(8):e24312. doi: 10.1371/journal.pone.0024312

Figure 5. Effect of rescorvitine (Rosc) on nocodazole (Noco)-induced prometaphase arrest in MCF-7 cells.

Figure 5

A (upper panel). Cells were pre-treated for 2 h with roscovitine (10, 20, and 30 µM) and then stimulated for additional 12 h with 250 nM nocodazole. Total cell lysates were analyzed by Western immunoblotting for cyclin B1. A (lower panel). The relative protein levels cyclin B1 are calculated according to their densitometry readings, which are normalized according to the corresponding readings for the GAPDH protein bands. Each value is mean ± S.D. from three replicate measurements. ** P<0.01 versus vehicle-treated control; # P<0.05, ## P<0.01 versus nocodazole treatment. B. Cells were pre-treated for 2 h with roscovitine (10, 20, and 30 µM) and then stimulated for additional 12 h with 250 nM nocodazole. The DNA content of cells was analyzed using flow cytometry as described in the Material and Methods section. C. Nuclei were stained with Hoechst-33342, and examined using a fluorescence microscope for prometaphase cells (original magnification, ×200). D. Quantitative data on prometaphase-arrested cells. Each bar is a mean ± S.D. value from three separate experiments. ** P<0.01 versus the vehicle-treated control; ## P<0.01 versus nocodazole treatment.