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Osteoclast differentiation in mouse bone marrow cultures is not affected by ghrelin. Bone marrow cells were cultured for 7 days with experimental substances or vehicle. Cells were fixed and stained and the number of TRAP positive cells with three nuclei or more was determined in each well. MNC: multinucleated cells, OPG: osteoprotegerin (positive control). Data are mean ± SEM; *indicates P < 0.05 compared to untreated control.
