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Ghrelin is weakly mitogenic to primary rat osteoblasts. Osteoblasts cultured from rat calvariae were growth-arrested in 0.1% BSA for 24 hours. Fresh media and experimental compounds were then added for a further 24 hours. (a) Thymidine incorporation: cells were pulsed with 3H-thymidine for the last 6 hours of incubation (ANOVA P = 0.018). (b) Cell counts (ANOVA P = 0.076). Data are mean ± SEM; *indicates P < 0.05 compared to untreated control by t-test.
