Table 1.
This study | Method | Result |
---|---|---|
Primary tissue | ||
Human osteoblasts (n = 10) | Quantitative PCR | 3/10 positive |
Human bone marrow (n = 16) | 4/16 positive | |
Mouse bone marrow | Negative | |
Rat osteoblastic cells | Negative | |
Mouse hypothalamus | Positive | |
Cell lines | ||
Mouse osteoblastic MC3T3-E1 | Negative | |
Mouse macrophage RAW264.7 | Negative | |
| ||
Kim et al. [23] | ||
| ||
Primary Tissue | ||
Rat osteoblastic cells | Western blot | Positive |
Mouse osteoblastic cells | ||
Rat brain | ||
Cell lines | ||
Mouse osteoblastic MC3T3-E1 | Positive | |
Mouse preadipocytic 3T3-L1 | RT-PCR, Western blot | |
Rat ROS17/2.8 osteogenic sarcoma | ||
Rat UMR-106 osteogenic sarcoma | ||
| ||
Maccarinelli et al. [24] | ||
| ||
Primary Tissue | ||
Rat osteoblastic cells | RT-PCR, Western blot | Positive |
| ||
Ueberberg et al. [25] | ||
| ||
Primary Tissue | ||
Human bone marrow | Quantitative PCR, IHC | Positive |
| ||
Delhanty et al. [27] | ||
| ||
Primary Tissue | ||
Human bone marrow (n = 2) | Quantitative PCR | Positive |
| ||
Fukushima et al. [28] | ||
| ||
Primary Tissue | ||
Rat osteoblastic cells | RT-PCR, IHC | Positive |
Rat femur | IHC | Positive |
Cell lines | ||
Rat UMR-106 osteogenic sarcoma | RT-PCR | Positive |
| ||
Guan et al. [35] | ||
| ||
Primary Tissue | ||
Rat bone marrow | RPA | Negative |
RT-PCR: reverse transcription PCR. IHC: immunohistochemistry. RPA, ribonuclease protection assay.