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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Aug 14;18(9):1015–1019. doi: 10.1038/nsmb.2105

Figure 1.

Figure 1

Cdk1 regulates long range DSB resection by Dna2.

(a) Analysis of initial and extensive resection in mutants with either active or inactive Cdk1 kinase. The Southern blots corresponding to these experiments are presented in Supplemental Figure 1. Error bars correspond to s.d. (b) Southern blot analysis of initial resection in mutants. Smearing and additional bands below the HO cut band typical for mutants that lack extensive resection are indicated by asterisks. (c) Analysis of resection in cells where Cdk1 activity is blocked at 4 hours after break induction when all cells have initiated resection. (d) Recruitment of Dna2 to DSBs was monitored in indicated mutants by ChIP. Error bars correspond to s.d.