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Dna2 is phosphorylated by Cdk1 and Mec1.
(a) Western blot analysis of Dna2-9×Myc phosphorylation in cdk1-as1 with or without Cdk1 inhibitor and in checkpoint deficient cells in response to a single DSB. (b) In vitro phosphorylation of wild-type Dna2 or dna2 mutant proteins lacking single or multiple Cdk1 phosphorylation consensus sites. (c) Western blot analysis of Dna2 phosphorylation in wild type cells and indicated dna2 mutants cells.
