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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Nat Struct Mol Biol. 2011 Aug 14;18(9):1015–1019. doi: 10.1038/nsmb.2105

Figure 4.

Figure 4

Dna2 phosphorylation by Cdk1 is needed for its recruitment to DSBs.

(a) Analysis of DSB recruitment of GFP-tagged wild-type Dna2 and indicated mutant dna2 proteins. (b) Analysis of recruitment of FLAG-tagged wild-type Dna2 and indicated mutant dna2 proteins to DSB ends by ChIP using primers specific for sequences located 1 kb upstream of the DSB. Error bars correspond to s.d. (c) Analysis of recruitment of GFP-tagged phosphomimic dna2S17D S237D protein to DSB ends in Cdk1 and Ku deficient cells.