Figure 3.

A soluble factor secreted from astrocytes mediates β-amyloid (Aβ)-induced neuronal death. Bar charts show lactate dehydrogenase (LDH) release into culture medium from rat primary neuronal cultures exposed to (a) conditioned medium from primary astrocyte cultures treated with 10 μM Aβ and/or 20 μM Min, and (b) cell-free primary astrocyte culture medium infused with 10 μM Ab for 48 h, with and without the addition of 20 μM minocycline (Min). Values are LDH release from cells into medium expressed as a percentage of total LDH, shown as mean±S.E.M. Experiments were performed in triplicate (N=9). ^*P<0.05; ^***P<0.001