Figure 4.

Astrocytes increase β-amyloid (Aβ)-induced caspase-3 activity. Western blots of cell lysates from rat primary mixed (M) and neuronal (N) cultures. Cultures were exposed to 10 μM Aβ for 48 h with and without pretreatment with 20 μM minocycline for 24 h. (a) Representative western blots probed with an antibody against cleaved caspase-3 (17 and 19 kDa). Blots were also probed with β-actin (42 kDa) as a loading control. Molecular weight markers are indicated (kDa). (b) Bar chart showing the amount of cleaved caspase-3 following standardisation to β-actin in each sample. Data are mean±S.E.M. Experiments were performed in triplicate (N=12). ^*P<0.05; ^**P<0.01; ^***P<0.001