Figure 6.

Astrocytes are necessary for β-amyloid (Aβ)-induced tau phosphorylation. Western blots of cell lysates from rat primary mixed (M) and neuronal (N) cultures treated with 10 μM Aβ for 48 h±20 μM minocycline (Min). (a) Representative western blots probed with antibodies recognising tau phosphorylated at Ser396/404 (PHF1) and Ser202 (CP13), and total tau. (b) Bar chart shows the amount of tau phosphorylated at either Ser396/404 (PHF1) or Ser202 (CP13) as a proportion of total tau. (c) Representative western blots of cell lysates from Aβ-treated mixed cultures and neuronal cultures, probed with antibodies recognising tau phosphorylated at Ser396 (green) and caspase-cleaved tau (red). Molecular weight markers are indicated (kDa). Data are mean±S.E.M. Experiments were performed in triplicate (N=12). ^*P<0.05; ^**P<0.01; ^***P<0.001