Figure 7.

 DRIP100, a novel protein that makes up part of a larger, ligand-dependent coactivator complex. (A) Primary sequence of DRIP100. Boxed residues denote a LXXLL signature motif previously identified as a short region that confers interaction with steroid/nuclear hormone receptors (Heery et al. 1997; Torchia et al. 1997). Note also several leucine doublets that are nonconsensus but nevertheless might be functional motifs (Heery et al. 1997). (B) Multiple tissue Northern blot of DRIP100. Two micrograms each of poly(A)⁺ RNA from the indicated human tissues (lanes 1–8) and from two cell lines (lanes 9,10) were probed with a radiolabeled 2.7-kb fragment of DRIP100 cDNA. (Arrows) One major and two minor RNA species; the predominant species is ∼4 kb. The blot was stripped and reprobed with a β-actin DNA fragment (shown directly below the DRIP100 blot).