Figure 8.

 Effect of overexpression of DRIP100 on VDR transactivation in HeLa cells. (A) In each transfection, 5.0 μg of (VDRE)×2–E1B–LUC reporter, and 0–4.0 μg of CMV–DRIP100 DNA was used. Each transfection was carried out without (EtOH) or with 10⁻⁸ m 1,25(OH)₂D₃ and 2.0 μg of a normalizing reporter, CMV–βgal. Transactivation was from endogenous VDR. The amount of CMV expression plasmids was kept constant by balancing the total amount with empty CMV vector (pRc-CMV). Quantitation is from a representative experiment done in triplicate, although the same trend was observed in multiple transfections. (B) Fold induction in response to 1,25(OH)₂D₃ (ratios of +ligand/−ligand taken from A).