Figure 5.

 The subcellular localization of Staufen requires Miranda. Miranda localizes normally throughout the cell cycle in staufen^D3 mutants (a,d,g,j; Miranda labeled in green, DNA in red). Staufen subcellular localization, however, is absolutely dependent on Miranda (b,e,h,k; Staufen labeled in green, Prospero in blue, DNA in red). In miranda^RR127, Staufen is appropriately localized, but concentrates more strongly at the apical side of the neuroblast at interphase (arrowhead in c; Staufen labeled in green, Prospero in blue, DNA in red), and forms a weaker basal crescent at mitosis (i,l). miranda mRNA (labeled in red, DNA in green) localizes predominantly on the apical side of the neuroblast throughout the cell cycle in both wild-type (m) and staufen^D3 embryos (n).