Figure 3.
Loss of insc causes RP2sib to RP2 and pCC to aCC fate transformations. Ventral view of whole-mount stage 15 embryos. Anterior is up. (A,C) Double staining with rabbit anti-Eve (red) and mouse anti-Zfh-1 (green). (B,D) Double staining with rabbit anti-Eve (red) and mouse mAb 22C10 (green). (A,B) wt: RP2 (arrow) and aCC (arrowheads) coexpress Eve and Zfh-1 (yellow in A) and form a 22C10-positive, ipsilateral axon projection (green in B); pCC (asterisk) neither expresses Zfh-1 (A) nor 22C10 (B). (C,D) insc22. Note that both GMC1-1a and GMC4-2a progeny coexpress Eve and Zfh-1 (yellow in C) and form an ipsilateral axon projection (D).