Figure 5.

insc is required for the coordination of Numb localization and the orientation of the metaphase plate in GMCs. Lateral views of dividing GMCs in stage 11 embryos triple stained with anti-Numb (with rhodamine conjugated secondary antibody, red), mouse anti-Eve (with FITC conjugated secondary antibody, blue) and DNA stain (green). Basal (dorsal) is up. (A,B,E,I, left) Wild type; (C,D,G,H,I, right) insc²². Top panels (A–D) depict the anti-Numb and anti-Eve staining; middle panels (E–H) show the anti-Numb and DNA staining of the same samples; bottom panels (I,J) show the anti-Numb and DNA staining in randomly chosen GMCs. (A,B,E,F,I) wt. Note the basal Numb crescents in all samples and the horizontal orientation of the metaphase plates in E and I. (C,D,G,H,J) insc²². Note the cortical Numb staining with no distinct crescents in C,G,J and the apical crescent in D and H. The metaphase plates are not oriented horizontally with respect to the apical surface (G,J). Relevant cells are circled with dots.