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. 1998 Jun 15;12(12):1775–1780. doi: 10.1101/gad.12.12.1775

Figure 3.

Figure 3

 mSiah2 is a nuclear receptor antirepressor. (A) Transfection of mSiah2 and Sina inhibits RevErb repression in 293T cells. Cells were transfected with RevErb expression vector and RevDR2–SV40–luciferase reporter as described previously (Harding and Lazar 1995). (B) mSiah2ΔN does not inhibit RevErb repression in 293T cells. Five micrograms of mSiah2 or mSiah2ΔN expression plasmid was cotransfected where indicated in A and B. (C) Sina also functions as antirepressor for Gal4–N-CoR (1–160) but not Gal4–SMRT(1–483). (D) mSiah2 partially blocks TR repression in 293T cells. Cells were transfected with (Gal4 × 5)–SV40–luciferase and Gal4–TR as described previously (Zhang et al. 1997). (E) MG132 blocks mSiah2 effects on RevErb repression in 293T cells. mSiah2 inhibits RevErb repression in a concentration-dependent manner, and this is prevented by treatment with MG132.