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. 1998 Jun 15;12(12):1801–1811. doi: 10.1101/gad.12.12.1801

Figure 3.

Figure 3

 κ chain demethylation in B-cell development. (a) Schematic diagram of murine B-cell development (adapted from Nutt et al. 1997). The different developmental stages of B lymphopoiesis are shown together with their characteristic cell surface markers, which are used for classification according to Rolink et al. (1994) (top) or Hardy et al. (1991) (bottom). As the correlation between the two classification systems is not straightforward in all aspects, the reader is referred to the original literature for details. (b) DNAs from bone marrow B220+ cells at different stages of B-cell development (see the diagram) were tested for methylation by digestion with HhaI (+). B-cell DNA from Rag1−/− mice (arrested at the pro-B stage) (Mombaerts et al. 1992) and from Rag1−/− mice carrying a μ transgene (arrested at the small pre-B stage) (Spanopoulou et al. 1994) was digested by HindIII with (+) or without (−) HhaI. DNA from wild-type (wt) fraction D cells (pre-B stage, Hardy et al. 1991; Ehlich et al. 1993) was analyzed by digestion using HindIII–BglII with (+) or without (−) HhaI.