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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Biochem J. 2011 Apr 15;435(2):327–336. doi: 10.1042/BJ20102118

Figure 1. Effect of inhibitors of fatty acid metabolism on hippocampal development.

Figure 1

Although indomethacin (Indo, 50 µM) and NDGA (10 µM) exerted no effects, inhibition of FAAH using URB597 (1 µM, FAAHI) enhanced neurite growth at 3 and 7 DIV (days in in vitro culture) (B) as well as synapsin puncta formation (C) and NMDA receptor expression at 7 DIV (D). (A) Representative photomicrographs of E18 mouse hippocampal neurons after culturing for 7 days in the presence of various inhibitors: MAP2 (a neuron-marker protein) green; synapsin-1, red; and DAPI (for nuclei), blue. Scale bars, 30 µm. (B and C) Quantitative changes in neurite growth (B) and synaptogenesis evaluated by the number of synapsin puncta/10 µm of neurite (C). Statistical analysis was performed by post-hoc Tukey’s HSD tests at the significance level of P < 0.05. Different alphabetical letters indicate statistically significant differences. (D) Western blot analysis for NR2B expression.