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. Author manuscript; available in PMC: 2012 Apr 15.
Published in final edited form as: Biochem J. 2011 Apr 15;435(2):327–336. doi: 10.1042/BJ20102118

Figure 2. Biotransformation of DHA into DEA in hippocampal neuronal cultures.

Figure 2

MS/MS spectra from [M+H]+ of 50 fmol of d4-labelled DEA internal standard (A), DEA standard (B) or 13C22[DEA] produced in E18 hippocampal neuronal cultures after supplementation with 1 µM [13C22]DHA (C) for 3 days. The characteristic fragmentation pattern indicated the production of [13C22]DEA in the hippocampal culture. The MS/MS spectrum of DEA produced from DHA in the culture was virtually identical with that of the standard DEA.