Nuclei composing the caudal NTS are indicated in A and D. A–C show the same section ~ 300 μm caudal to obex where the commissural nucleus of the solitary tract (SolC) is most prominent. D–F show a single section ~ 400 μm rostral to obex where the area postrema is near its maximal mediolateral extent.
In A and D red neurons are immunofluorescent for neuron specific nuclear protein (NeuN). In A, B, D and E labeling in the dorsal motor nucleus of the vagus (10N), hypoglossal nucleus (12N), area postrema (AP) and subpostrema nucleus (SubP) resulted from a subcutaneous injection of FluoroGold. In C and F darkfield illumination identifies the myelinated fascicles of the solitary tract (sol).
A and D are true color RGB images captured using a triple band-pass epifluorescence filter (uv/DAPI, FITC, Texas Red) and a RGB liquid crystal filter. Images in B and E were extracted from the green channel of the RGB images in A and D respectively.