Skip to main content
. 2011 Aug 22;108(36):14986–14991. doi: 10.1073/pnas.1109180108

Fig. 3.

Fig. 3.

Assessment of AF2ER function in the AF2ERKI mouse uterus. (A) Uterine wet weight after vehicle (Veh), ICI (2 mg/kg), TAM (2 mg/kg), E2 10 μg/kg, or E2 2 mg/kg treatments for three consecutive days. Values are presented as mean ± SEM. (B) Uterine histology after vehicle, ICI, TAM, or E2 (10 μg/kg) treatments for three consecutive days in representative mice. (C) ICI and TAM induce the proliferation of endometrial epithelial cells in AF2ERKI uterus. Uterine EdU incorporation was analyzed. Hoechst was used as a counterstain to visualize tissue. (D) The mRNA levels of Ltf, Igf1, and Cox7a2l genes were quantified by real-time PCR. The mRNA levels were represented as fold change vs. vehicle treatment of WT. Values are shown as mean ± SD; *P < 0.05 vs. vehicle in each genotype. (E) Representative results of Western blots probed for ERα and β-tubulin from the vehicle- and ICI-treated individual mouse uteri are shown. The ERα level was normalized to the level of β-tubulin in each sample and presented as fold change vs. vehicle in each group.