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. 1998 Jun 15;12(12):1763–1768. doi: 10.1101/gad.12.12.1763

Figure 1.

Figure 1

Cre–loxP-mediated targeting of the Ipf1/Pdx1 gene. (A) Schematic representation of targeting construct, genomic DNA, and the expected product of Cre/loxP recombination. The PCR primers used for genotyping and the probes used for confirmation of correct recombination events are shown. (B) RT–PCR analysis using Cre- and rpS17 control primers on total RNA prepared from the alimentary tract of E10.5, E12.5, and E14.5 Rip1/Ipf1▴ and E14.5 Ipf1+/+ (ctrl) embryos. Pancreatic sections from neonatal (C), 3 (D)-, and 5 (E)-week-old prediabetic Rip1/Ipf1▴ mice stained with anti-Ins and anti-IPF1 antibodies. (F–O) β-Cell-specific loss of Ipf1/Pdx1 affects the β-cell phenotype. Confocal images showing Ipf1+/+ (F,H,J,L,N) and diabetic Rip1/Ipf1▴ (G,I,K,M,O) islets from 18-week-old mice. See text for details. (C–O) Bar, 10 μm.