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Identification of different oligomerization states of the recombinant hMAD2 protein. Oligomerization states of the recombinant hMAD2 proteins were determined by gel filtration chromatography with a S100 column. Column fractions were analyzed by SDS-PAGE. The elution peaks of molecular mass standards are labeled at the top of the UV trace. (*) Peak corresponding to void volume. (WT) Wild-type hMAD2; (ΔN) hMAD2ΔN; (ΔC) hMAD2ΔC.
