hMAD2 and hCDC20 colocalize to kinetochores at prometaphase. (A) HeLa cells were stained with anti-hMAD2, CREST, and DAPI. (B) Hela cells were stained with anti-hCDC20, CREST, and DAPI. (C) Hela cells were stained with anti-hCDC20, anti-hMAD2, and DAPI.