Fig. 3.

AP4 modulates variegated CD4 derepression in mice harboring a mutated Cd4 silencer. (A) CD4 and CD8 expression in TCRβ^hiHSA^lo gated mature thymocytes from Tcfap4^−/− mice crossed to Cd4 silencer site 3 mutant mice. CD4 expression in site 3 mutant CD8⁺ mature thymocytes on either AP4-sufficient (Upper) or -deficient (Lower) backgrounds is shown (Right). Filled histograms show CD4 expression in CD8⁺ mature thymocytes from AP4-sufficient (Upper) or -deficient (Lower) mice with the intact Cd4 silencer. (B and C) CD4 expression in CD8⁺TCRβ⁺ lymph node (LN) cells of mice with silencer site 3 mutation, AP4 deficiency, or both. A representative set of data is shown in B and a statistical analysis of frequency of CD4⁺ T cells within the CD8⁺ T-cell interval gate in multiple mice is shown in C. Averages and SDs are shown with P values determined by unpaired Student's t test.