Changes in CD4 expression patterns in different subsets of CD8+ T cells harboring mutated Cd4 silencers. (A) CD4 expression in total CD8+ T cells, CD8+CD103+ and CD8+CD103– subsets from WT (filled histograms), Cd4 silencer-deficient, and Cd4 silencer site 1 mutant (open histograms) mice. Frequencies of CD4+ cells are shown within the interval gates. (B) CD4 expression in CD8+ T-cell subsets from Cd4 silencer site 1 mutant, AP4-sufficient (Upper), or -deficient (Lower) mice. (C) Statistical analysis by unpaired Student's t test of the percentages of CD4+ cells in different subsets of site 1 mutant CD8+ T cells.