Fig. 5.

AP4 binding to the Cd4 proximal enhancer and interaction with Runx protein. (A) Chromatin immunoprecipitation analysis showing AP4 binding to the Cd4 proximal enhancer in double-negative thymocytes. Results are representative of four independent experiments. (B) H3K9ac (Left) and H3K27ac (Right) histone modifications at Cd4 regulatory elements in DN thymocytes. Data are representative of two independent experiments. (C) Physical interaction of endogenous AP4 and Runx1 in 1200M cells. Immunoprecipitation (IP) with anti-AP4 (Upper) or anti-Runx (Lower) antibodies was followed by immunoblotting with antibodies specific for the two transcription factors. Results are representative of three independent experiments. WB, Western blot.