Fig. 7.

Effect of AT₁R blocker losartan on expression of mitochondrial AT₁Rs and AT₂Rs. Renal tubular cell sections from C57BL/6 20 wks old (A and E), 70 wks old (B and F), and 70 wks old treated with losartan 40-60 mg/kg/day for 20 wks (C and G). D and H represent average counts of immune-labeling densities of mtAT₁R (D) and mtAT₂R (H) by age group in response to losartan. Gold particles in 30 mitochondria from each immunolabeling experiment were counted and averaged. Age was associated with a significant decrease in mtAT₂R that was reversed with chronic use of losartan. *P < 0.005, **P < 0.0005.