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. 1998 Jun 15;12(12):1884–1893. doi: 10.1101/gad.12.12.1884

Figure 1.

Figure 1

 (A) Sequence of the nifA1 promoter region and engineered promoters. (Shaded bars) RcNtrC binding sites, (arrowheads) major DNase I hypersensitive sites. The −35 and −10 hexamers are underlined and the transcriptional start site is marked by a horizontal arrow. The sequences of the nifA1, nifA1Mut1, nifA1Mut2, and nifA1Mut3 promoters are aligned for comparison with the E. coli σ70 consensus. (B) In vitro transcriptional activation of nifA1 wild-type and mutant promoters by RcNtrC. Templates are noted below each set of transcription reactions. (Lanes 1,6,11,16) No activators; (lanes 2,7,12,17) 260 nm RcNtrCC3; (lanes 3,8,13,18) 260 nm RcNtrCC3 and 270 nm MBP–NTRB; (lanes 4,9,14,19) 350 nm RcNtrCC7; (lanes 5,10,15,20) 350 nm RcNtrCC7 and 270 nm MBP–NtrB.