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Purified σ70 added to R. capsulatus core RNAP stimulates transcriptional activation by RcNtrC. In vitro transcription reactions using the supercoiled nifA1Mut1 template with the R. capsulatus core RNAP (lanes 1,2) or core RNAP with the addition of His-tagged σ70 subunit (lanes 3,4) or SDS-gel purified σ70 subunit (lane 5). (Lanes 1,3) no activator; (lanes 2,4,5) 270 nm MBP–NtrB and 260 nm RcNtrCC3.
