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. 1998 Jun 15;12(12):1917–1928. doi: 10.1101/gad.12.12.1917

Figure 1.

Figure 1

 Expression of the C/EBPβ isoforms in C57/Bl6 mice during mammary development. (A) Mammary tissue was isolated from mice at the following developmental stages: immature virgin (6 week vir), mature virgin (12 week vir), 6 days of pregnancy (6-P), 10 days of pregnancy (10-P), 15 days of pregnancy (15-P), 18 days of pregnancy (18-P), 2 days of lactation (2-L), 10 days of lactation (10-L), and 10 days of lactation followed by 4 days of involution induced by removal of pups (Inv). Aliquots of WCE (100 μg) were separated by SDS-PAGE, transferred to membrane, and probed with antibodies against rat C/EBPβ (Santa Cruz, sc-150-x) as described in Materials and Methods. The LAP2 (36 kD) and LIP (20 kD) isoforms of C/EBPβ are shown (A,B). As a positive control for expression of LIP (A), an aliquot (100 μg) from a late pregnant WAP-driven LIP transgenic mouse (WAP–LIP) was included. (B) The 45-kD band referred to previously as LAP1 (Raught et al. 1995) is CRM as indicated by probing C/EBPβ−/− virgin mg (−/−mg) with C/EBPβ antibody. In addition, CRM at 30 kD was noted in some C/EBPβ−/− mammary gland extracts and in the rat liver nuclear extract (rLNE) included as a positive control (B).