Figure 5.

Salt- and ethanol shocks result in the export of Npl3-27p. Both double-mutant strains xpo1-1 npl3-27 (top two rows) and rat7-1 npl3-27 (bottom two rows) were grown to log phase and shifted to 37°C for 30 min before they were stressed with 0.4 m NaCl for 10 min (left) or with 10% ethanol for 30 min (right). Cells were then prepared for immunofluorescence (IF) with α-Npl3p (first and third row) and a dT probe (second and fourth row).