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. 1999 Aug 1;13(15):1994–2004. doi: 10.1101/gad.13.15.1994

Figure 6.

Figure 6

Figure 6

UV cross-linking of Npl3p to poly(A)+ RNA. (Top) Cells were either treated with or without high salt (+ or − Salt shock) and exposed to UV for cross-linking (+ or −UV-irradiation). The lysates were taken before application to the oligo(dT)–cellulose-column. (Flow through) The material that did not bind to the column. The final wash is derived from the last wash before elution (see Materials and Methods). The eluate contains the cross-linked poly(A)+ RNA-associated proteins. (Bottom) Cells were either shifted to 42°C for 15 min and exposed to UV (+heat shock +UV) or UV cross-linked without a heat shock (− heat shock +UV). The immunoblot was carried out with a polyclonal antibody against Npl3p.