Figure 8.

TRIP8b is required for expression of dendritic HCN channel gradients. A, Immunostaining reveals loss of HCN1 enrichment in TRIP8b^−/− layer V neocortical pyramidal neuron dendrites. Scale bar, 50 μm. B, Hippocampal immunostaining for HCN1 or HCN2 (green) demonstrates loss of distal enrichment and decreased protein levels in TRIP8b^−/− mice (**p < 0.01, ***p < 0.001, n = 3, unpaired t test). Regions are denoted by drawing a straight line perpendicular from the stratum pyramidale toward the apical (containing SR and SLM) or basal dendritic fields (containing SO), and dividing them into equal length sections. SO was divided into two sections, SP was divided into one section, SR was divided into seven sections, and SLM was divided into three sections. This method was adapted from the study by Shin and Chetkovich (2007). Scale bar, 400 μm.