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. 1999 Aug 1;13(15):2005–2016. doi: 10.1101/gad.13.15.2005

Figure 5.

Figure 5

The DNA strand exchange in trans requires an active RecA nucleoprotein filament. Presynaptic filaments were assembled by incubation of RecA protein (1.3 μm) with ssDNA (#43.4 μm) in the presence of 1 mm ATPγS (█), 5 mm ADP/10 mm NaF/0.4 mm Al(NO3)3 (●), 5 mm ADP (○), or in the absence of cofactor (▴) under standard conditions. For each filament, the DNA strand exchange in trans was initiated by addition of a pair of mutually homologous DNA substrates: dsDNA (#5 and #6; 8 μm) and 32P-labeled ssDNA (#5; 4 μm).