Figure 9.
The heteroduplex dsDNA product of DNA strand exchange in trans is associated weakly with the RecA nucleoprotein filament. For the reactions in trans, presynaptic filaments were assembled on ssDNA (#43); these filaments were mixed with dsDNA (#5 and #6) and 32P-labeled ssDNA (#5). For the reactions in cis, the presynaptic filaments were formed with 32P-labeled ssDNA (#5) and mixed with homologous dsDNA (#5 and #6) in the presence of heterologous ssDNA (#43). Both reactions were carried out at 37°C. The products of these two reactions were resolved on a polyacrylamide gel, both as native complexes (A) and after deproteinization (B).