Table 1.
Cell fate in gsk-3(RNAi) embryos
| A. Polarizing opticsa | |
|---|---|
| Percent producing endoderm (total) | |
| Wild type intact | 100 (50/50) |
| Wild type isolated E | 100 (32/32) |
| Wild type isolated MS | 0 (0/10) |
| Wild type isolated C | 0 (0/16) |
| Wild type C and E ablated | 0 (0/6) |
| gsk-3(RNAi) | 82 (300/364) |
| gsk-3(RNAi) isolated E | 56 (14/25) |
| gsk-3(RNAi) isolated MS | 0 (0/8) |
| gsk-3(RNAi) isolated C | 71 (15/21) |
| gsk-3(RNAi) C and E ablated | 0 (0/8) |
| B. Tissue productionb | ||
|---|---|---|
| Percent making pharyngeal muscle | Percent making body wall muscle | |
| Wild type isolated E | 0 (0/9) | 0 |
| gsk-3(RNAi) isolated E | 30 (4/12)* | 38 (3/8)* |
a
Using polarizing optics to detect the presence of rhabditin granules, endoderm was scored in terminally differentiated embryos following ablation of indicated blastomeres. The total number of ablations scored is indicated in parenthesis.
b
Tissue production was scored using tissue-specific antibodies on terminally differentiated, fixed embryos following ablation of indicated blastomeres. Embryos were stained with 3NB12, a monoclonal antibody that recognizes pharyngeal muscle cells, or 5.6, one that recognizes body wall muscle. An asterisk indicates that all positive staining occurred only in embryos that did not make endoderm.