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. 1999 Aug 1;13(15):1918–1923. doi: 10.1101/gad.13.15.1918

Figure 1.

Figure 1

Figure 1

Figure 1

Targeted disruption of the mouse Daxx gene. (A) A map of the mouse genomic Daxx locus. The seven exons are indicated as open boxes. The targeting vector includes a thymidine kinase (TK) gene for negative selection and a neo gene for positive selection. Homologous recombination and insertion of neo results in a 2.5-kb deletion including exon 1 and most of exon 2. The positions of an external probe from 5′ of Daxx and of an internal cDNA probe used for genotyping HindIII-digested DNA by Southern blot analysis are shown. (B) BamHI; (E) EcoRI; (H) HindIII; (K) KpnI; (S) SacI; (Sal) SalI; (X) XhoI; (Xb) XbaI. Not all K and S sites are shown. (B) PCR analysis of embryo yolk sac genomic DNA. (C) Northern blot analysis of total RNA from blastocyst-derived cell lines. A cDNA probe, generated by PCR and encompassing bp 1596–2274, detects a wild-type band of 2.4 kb. In mutant cells, a stable transcript of 1.4 kb is detected.