Figure 5.

Daxx is localized to the nucleus. (A) Total cell extracts were prepared from wild-type and Daxx-deficient cell lines. Total cell lysate (30 μg) or cell lysate that had been immunoprecipitated with α-Daxx (1.5 mg), was loaded on an 8% SDS–polyacrylamide gel. α-Daxx was used for immunoblotting. (B) Wild-type ES cells were fractionated into the following subcellular components: nucleus (n); membrane (mb); and cytosol (c). Immunoprecipitation and immunoblotting were performed with α-Daxx. (C) HeLa nuclear extracts were used for immunoprecipitation with α-Daxx or α-CAP. α-Daxx was used for immunoblotting. (D) Immunoblotting of subcellular fractions was performed with an antibody recognizing laminin B.