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. 1999 Aug 1;13(15):1983–1993. doi: 10.1101/gad.13.15.1983

Figure 2.

Figure 2

prp8 alleles suppress mutations at the 5′SS and 3′SS in vivo. (A) Copper growth phenotypes of wild-type and selected prp8 suppressor strains in the presence of ACT1–CUP1 reporters containing 5′SS and 3′SS mutations, as indicated. (B) Primer extension analysis of in vivo splicing of ACT1–CUP1 reporter containing selected 5′SS and 3′SS mutations. Extension products generated with wild-type PRP8 (lanes 1,2,11,13,15,17) or prp8 suppressors (lanes 3–10,12,14,16,18, as indicated) in the presence of wild-type (lane 1), U+2A (lanes 2–12), U+2G (lanes 13,14), UAG →  UUG (lanes 15,16), or UAG → GAG (lanes 17,18) reporters were resolved on a 7% polyacrylamide/8 m urea gel. Positions of U14 snRNA internal control, pre-mRNA, mRNA, and lariat intermediate products are indicated. Note that sequence differences among various reporters result in distinct mobilities of their extension products.