Table 2.
Phenotypes of prp8-151-7 alleles—primer extension analysis
| Prp8/reporter/U1
|
%P
|
%L
|
%M
|
M+L/P (step 1)
|
M/L (step 2)
|
|---|---|---|---|---|---|
| wt/U2A/− | 84.1 ± 1.8 | 10.5 ± 1.3 | 5.4 ± 1.5 | 1.00 | 1.00 |
| 151/U2A/− | 85.2 ± 0.4 | 4.9 ± 0.3 | 9.9 ± 0.3 | 0.92 ± 0.3 | 3.93 ± 0.2 |
| 152/U2A/− | 85.2 ± 1.6 | 4.0 ± 0.4 | 10.9 ± 1.3 | 0.91 ± 0.2 | 5.34 ± 0.2 |
| 152b/U2A/− | 86.4 | 9.2 | 4.4 | 0.83 | 0.93 |
| 153/U2A/− | 84.6 ± 1.2 | 6.7 ± 1.1 | 8.7 ± 1.5 | 0.96 ± 0.1 | 2.60 ± 0.8 |
| 154/U2A/− | 84.4 ± 3.0 | 5.8 ± 1.4 | 9.8 ± 2.2 | 0.98 ± 0.2 | 3.29 ± 0.8 |
| 155/U2A/− | 82.6 ± 1.2 | 4.9 ± 0.9 | 12.5 ± 0.3 | 1.11 ± 0.1 | 4.70 ± 0.8 |
| 156/U2A/− | 83.4 ± 0.6 | 4.5 ± 0.8 | 12.1 ± 0.9 | 1.05 ± 0.05 | 5.23 ± 1.4 |
| 157/U2A/− | 86.3 ± 0.8 | 3.8 ± 0.4 | 10.0 ± 1.0 | 0.85 ± 0.05 | 5.12 ± 1.0 |
| wt/wt/− | 9.1 ± 1.8 | 4.4 ± 1.3 | 86.5 ± 2.0 | 52.84 ± 8.6 | 38.22 ± 9.0 |
| wt/U2A/U1–7U | 61.8 ± 2.8 | 27.2 ± 1.7 | 11.0 ± 1.4 | 3.27 ± 0.4 | 0.79 ± 0.1 |
| 151/U2A/U1–7U | 59.5 ± 0.3 | 11.5 ± 1.7 | 29.0 ± 2.0 | 3.60 ± 0.05 | 4.90 ± 0.1 |
| 152/U2A/U1–7U | 65.3 ± 3.8 | 12.8 ± 2.2 | 21.2 ± 2.4 | 2.81 ± 0.5 | 3.22 ± 1.4 |
| 153/U2A/U1–7U | 61.0 ± 3.0 | 15.2 ± 1.8 | 23.8 ± 1.6 | 3.38 ± 0.5 | 3.04 ± 0.4 |
| 154/U2A/U1–7U | 64.2 ± 3.2 | 12.7 ± 3.3 | 23.1 ± 0.7 | 2.95 ± 0.4 | 3.54 ± 0.8 |
| 155/U2A/U1–7U | 59.4 ± 3.3 | 14.6 ± 1.7 | 26.0 ± 2.8 | 3.51 ± 0.5 | 3.46 ± 0.6 |
| 156/U2A/U1–7U | 56.3 ± 5.3 | 8.7 ± 2.0 | 35.0 ± 4.4 | 4.11 ± 0.9 | 7.82 ± 1.9 |
| 157/U2A/U1–7U | 65.4 ± 3.9 | 6.8 ± 1.6 | 27.8 ± 3.4 | 2.80 ± 0.5 | 7.95 ± 1.9 |
| wt/wt/U1–7U | 7.8 ± 1.4 | 4.4 ± 1.2 | 87.8 ± 0.2 | 62.52 ± 12.6 | 38.80 ± 9.0 |
| wt/U2A/U1–wt | 88.2 ± 0.4 | 8.0 ± 0.1 | 3.9 ± 0.3 | 0.71 ± 0.02 | 0.95 ± 0.1 |
| 155/U2A/U1–wt | 87.3 ± 2.1 | 3.7 ± 0.9 | 9.0 ± 1.2 | 0.93 ± 0.1 | 4.35 ± 0.6 |
| 156/U2A/U1–wt | 81.0 ± 0.5 | 4.5 ± 0.1 | 14.8 ± 0.4 | 1.26 ± 0.04 | 6.40 ± 0.1 |
| 157/U2A/U1–wt | 85.3 ± 0.6 | 3.7 ± 0.1 | 11.0 ± 0.8 | 0.91 ± 0.05 | 5.78 ± 0.6 |
| wt/wt/wt | 5.7 ± 0.1 | 4.3 ± 2.0 | 90.0 ± 2.1 | 87.51 ± 1.2 | 40.70 ± 1.6 |
| wt/U2G/− | 86.5 ± 3.5 | 9.1 ± 2.7 | 4.5 ± 0.8 | 0.83 ± 0.3 | 0.96 ± 0.1 |
| 153/U2G/− | 91.9 ± 1.8 | 5.3 ± 1.7 | 2.9 ± 0.1 | 0.47 ± 0.1 | 1.06 ± 0.4 |
| wt/UUG/− | 36.5 | 37.5 | 26.0 | 1.00 | 1.00 |
| 153/UUG/− | 21.1 | 31.3 | 47.5 | 2.15 | 2.19 |
| wt/GAG/− | 23.4 | 34.9 | 41.7 | 1.00 | 1.00 |
| 153/GAG/− | 17.3 | 14.3 | 68.4 | 1.46 | 4.00 |
The identity of the prp8, ACT1–CUP1 reporter, and U1 snRNA genes is indicated in the first column. The second to fourth columns show percent distribution of pre-mRNA (P), lariat intermediate (L), and spliced exons (mRNA, M). The fifth and sixth columns display the efficiency of the first and second steps as M + L/P and M/L ratios, respectively. The primer extension data were obtained from 3–10 independent experiments (except for values provided without error bars, which represent a single experiment). The values in columns five and six are normalized to data obtained for the corresponding reporter in the presence of the wild-type PRP8, and in the absence of additional U1 snRNA genes.