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. 2011 Apr 14;(50):2357. doi: 10.3791/2357
Material Name Type Company Catalogue Number Comment
RPMI medium 1640 Reagent Gibco 21870-076  
Trypsin Reagent Worthington Biochemical Corporation LS003740 TPCK treated
Leibovitz's L-15 medium Reagent Gibco 41300-039  
Agarose, type I Reagent Sigma-Aldrich A6013 Use low melting temperature agarose, such as SeaPlaque agarose
Madin-Darby Canine Kidney cells Reagent ATCC CCL-34