Material Name | Type | Company | Catalogue Number | Comment |
RPMI medium 1640 | Reagent | Gibco | 21870-076 | |
Trypsin | Reagent | Worthington Biochemical Corporation | LS003740 | TPCK treated |
Leibovitz's L-15 medium | Reagent | Gibco | 41300-039 | |
Agarose, type I | Reagent | Sigma-Aldrich | A6013 | Use low melting temperature agarose, such as SeaPlaque agarose |
Madin-Darby Canine Kidney cells | Reagent | ATCC | CCL-34 |