Figure 7.

Effect of TNFα and glucocorticoids on promoter recruitment of pol II and the TF_IIH helicase subunit ERCC3. Chromatin immunoprecipitation assays were performed on A549-k9 cells by using (A) a pol II antibody (sc-899, Santa Cruz Biotechnology) or (B) a TF_IIH helicase subunit ERCC3 antibody (sc-293, Santa Cruz Biotechnology). Left panel (INPUT) shows that the starting chromatin extracts had equal amounts of the various chromatin regions; right panel (A, total pol II; B, ERCC3) shows enrichment of the interleukin-8 (IL-8) and ICAM-1 experimental regions; fold enrichment values for the experimental regions were determined by normalizing to the internal control U6 snRNA intensity.